Immunohistochemistry in HHV-6

This technique is useful in analyzing biopsy or autopsy sections. Cells from the tissue are stained with HHV-6 monoclonal antibodies to determine which proteins were expressed at the time the biopsy was taken. Pathologists can also differentiate A variant from B variant by using variant specific antibodies. Laboratory protocols for performing HHV-6 IHC on various tissue sample preparations have been developed by researchers and generously provided below:

  • Fresh/Frozen Samples (preferred)
    • A protocol from Dr. Irmeli Lautenschlager for performing IHC on fresh tissue samples is available for download here.
  • Paraffin-embedded samples
    • A protocol from Dr. Radhakrishna Sura for performing HHV-6 IHC on paraffin-embedded samples is available for download here. You may also see more about Dr. Sura’s protocol in this paper on HHV-6 and Crohn’s Disease.
    • A protocol from Dr. Gerhard Krueger for performing HHV-6 & HHV-7 IHC on paraffin-embedded samples is available for download here.

Induction of HHV-6 and HHV-7 in latently infected cell lines

A protocol from Dr. Janos Luka to induce viral replication in the inducible cell lines (stationary phase) of HHV-6 and HHV-7 latent cells is available for download here.

Protocol for High Titre virus stock preparation

A protocol for growing high titer purified virus stocks was written by Bhupesh Prusty, Phd, and can be downloaded here.