The CDC Procedure Manual for HHV-6 & 7 (1995)

The protocol book developed by Dr. Philip Pellett while at the CDC in 1995, including virus and cell preservation, slide preparation, IFA, anti-complement assay, immunoblot assay, Southern blot, hybridization, probe labelling, etc. Download here.

Immunohistochemistry in HHV-6

  • Fresh/Frozen samples
    • A protocol from Dr. Irmeli Lautenschlager for performing IHC on fresh tissue samples is available for download here.
  • Paraffin-embedded samples
    • A protocol from Dr. Radhakrishna Sura for performing HHV-6 IHC on paraffin-embedded samples is available for download here. Also see his paper on HHV-6 and Crohn’s Disease.
    • A protocol from Dr. Gerhard Krueger for performing HHV-6 & HHV-7 IHC on paraffin-embedded samples is available for download here.

Protocol for thawing and amplification of iciHHV6 cells immortalized with EBV

Induction of HHV-6 and HHV-7 in latently infected cell lines

  • A protocol from Dr. Janos Luka to induce viral replication in the inducible cell lines (stationary phase) of HHV-6 and HHV-7 latent cells is available for download here.

Protocols for high titer virus propagation

Protocol for GFP virus propogation and TCID50 for HHV-6A

Shared by Dr. Eain Murphy

For additional information on culturing and isolation, please review our FAQ.