The following cloned fragments of HHV-6 are available through the HHV-6 Foundation Repository either as bacterial stabs for growing your own DNA or as purified DNA (~10µg/vial):
|Name||Vector||Insert (Map on HHV-6 genome)||Insert size (kB)||Remarks|
|pMF147-19||pWE15-cosmid||90-130||~40||See Reference 1|
|pMF147-31||pWE15-cosmid||15-52||~27||See Reference 1|
|pMF210-8||pWE15-cosmid||72-110||~38||See Reference 1|
|pMF228-14||Bluescript KS-(BamHI)||2-30||~28||See Reference 1, See Note 1|
|pMF402-27||pCIN cosmid (SmaI)||112-155||~43||See note 2|
1. Frank Neipel, Klaus Ellinger, Bernhard Fleckenstein. The unique region of the human Herpesvirus-6 is essentially collinear with the UL segment of human cytomegalovirus. J. General Virology (1991) 72. 2293-2297
2. Martin ME, Thomson BJ, Honess RW, Craxton MA, Gompels UA, Liu MY, Littler E, Arrand JR, Teo I, Jones MD. The genome of human Herpesvirus 6: maps of unit-length and concatemeric genomes for nine restriction endonucleases. J. General Virology (1991) 72-157-168.
Note 1: pMF228-14 under non-optimal hybridization conditions may hybridize non-specifically to human chromosomes.
Note 2: pMF402-27 is a replacement clone of pMF 2200-21 described in Reference 1 which was unstable. The pMF402-27 clone has a small deletion in the region described as unstable by Martin, et al. (Reference 2).