The following cloned fragments of HHV-6 are available through the HHV-6 Foundation Repository either as bacterial stabs for growing your own DNA or as purified DNA (~10µg/vial):
Name | Vector | Insert (Map on HHV-6 genome) | Insert size (kB) | Remarks |
pMF147-19 | pWE15-cosmid | 90-130 | ~40 | See Reference 1 |
pMF147-31 | pWE15-cosmid | 15-52 | ~27 | See Reference 1 |
pMF210-8 | pWE15-cosmid | 72-110 | ~38 | See Reference 1 |
pMF228-14 | Bluescript KS-(BamHI) | 2-30 | ~28 | See Reference 1, See Note 1 |
pMF402-27 | pCIN cosmid (SmaI) | 112-155 | ~43 | See note 2 |
References:
1. Frank Neipel, Klaus Ellinger, Bernhard Fleckenstein. The unique region of the human Herpesvirus-6 is essentially collinear with the UL segment of human cytomegalovirus. J. General Virology (1991) 72. 2293-2297
2. Martin ME, Thomson BJ, Honess RW, Craxton MA, Gompels UA, Liu MY, Littler E, Arrand JR, Teo I, Jones MD. The genome of human Herpesvirus 6: maps of unit-length and concatemeric genomes for nine restriction endonucleases. J. General Virology (1991) 72-157-168.
Notes:
Note 1: pMF228-14 under non-optimal hybridization conditions may hybridize non-specifically to human chromosomes.
Note 2: pMF402-27 is a replacement clone of pMF 2200-21 described in Reference 1 which was unstable. The pMF402-27 clone has a small deletion in the region described as unstable by Martin, et al. (Reference 2).